Journal:

Article Title: Adenovirus Endocytosis Requires Actin Cytoskeleton Reorganization Mediated by Rho Family GTPases

doi:

Figure Lengend Snippet: Ad interaction with cells induces morphologic changes and reorganization of the actin cytoskeleton. Serum-starved A549 epithelial cells were incubated for various times with different ligands at 37°C, permeabilized, and stained for polymerized actin with rhodamine-labeled phalloidin. (A and G) Unstimulated control cells. (B and C) Cells incubated at 37°C for 5 to 10 min with 100 Ad2 particles/cell. (D) Cells incubated with Ad2 for 25 min at 37°C. (E and F) Cells incubated with 100 ng of EGF/ml for 5 min at 37°C. (H and I) Cells treated with 100 nM wortmannin followed by incubation with 100 Ad2 particles/cell for 10 min. Arrowheads indicate sites of membrane filopodia, while the arrows indicate sites of lamellipodia.

Article Snippet: Ad type 2 (Ad2) (American Type Culture Collection) was propagated in A549 cells and isolated by ultracentrifugation on CsCl density gradients as previously described ( 16 ).

Techniques: Incubation, Staining, Labeling

Journal:

Article Title: Adenovirus Endocytosis Requires Actin Cytoskeleton Reorganization Mediated by Rho Family GTPases

doi:

Figure Lengend Snippet: Disruption of cortical actin filaments with cytochalasin D inhibits Ad internalization. A549 cells were incubated in the presence of 0.5 μM (squares) or 2 μM (open circles) cytochalasin D at 37°C for 90 min or in medium containing 0.1% dimethyl sulfoxide (closed circles). Internalization of 125I-labeled Ad2 particles was subsequently measured by resistance to trypsin treatment as described in Materials and Methods.

Article Snippet: Ad type 2 (Ad2) (American Type Culture Collection) was propagated in A549 cells and isolated by ultracentrifugation on CsCl density gradients as previously described ( 16 ).

Techniques: Incubation, Labeling

Journal:

Article Title: Adenovirus Endocytosis Requires Actin Cytoskeleton Reorganization Mediated by Rho Family GTPases

doi:

Figure Lengend Snippet: Inhibition of Ad internalization by C. difficile toxin B. A549 cells were incubated for 5 h in serum-free medium containing various amounts of recombinant toxin B, a specific inhibitor of Rho family GTPases, prior to measuring Ad internalization by resistance to trypsin treatment at 10 min postwarming.

Article Snippet: Ad type 2 (Ad2) (American Type Culture Collection) was propagated in A549 cells and isolated by ultracentrifugation on CsCl density gradients as previously described ( 16 ).

Techniques: Inhibition, Incubation, Recombinant